Figure 7.
MACF1 regulates the number and positioning of hippocampal GABAergic interneurons. (A) Interneuron positioning patterns of control and MACF1loxP/loxP; Dlx5/6-CIE mice. Brain sections of control (MACF1loxP/+; Dlx5/6-CIE) and MACF1loxP/loxP; Dlx5/6-CIE mice at P14 were immunostained with an anti-GFP antibody. MACF1-mutant brains exhibited decreased interneurons in the hippocampus compared with controls. Scale bar, 250 and 20 μm. (B) Quantification of the number of interneurons in different areas of control and MACF1-mutant hippocampi. N = 1887 cells from 5 control mice, and N = 1589 cells from 5 MACF1-mutant mice. Statistical significance was determined by two-tailed Student's t-test. ***P < 0.001.