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. 2016 Oct 25;26(1):65–78. doi: 10.1093/hmg/ddw368

Figure 2.

Figure 2.

Lap1 knock out mouse skeletal muscle histology and whole-mount in situ hybridization. (A) Whole-mount in situ hybridization of E11.5 embryos of the indicated genotype with a myogenin probe. Note that myogenin positive signals are detected in somites. Scale bar: 500 µm (B) Percentage of myogenin positive somites among total somites. Values are means ± standard errors (n = 3 per genotype); **P < 0.001. (C) Photographs of Lap1+/+ and Lap1-/- mice at P2.5. (D) Representative hematoxylin and eosin-stained cross sections of quadriceps muscle from Lap1+/+ and Lap1-/- mice at P2.5. Scale bar: 25 µm (E) Quantification of individual quadriceps myofibre CSA from hematoxylin and eosin-stained cross sections from each genotype (n = 2 per genotype). Three different fields from each section from different animals were photographed and measured. A total of 311 myofibres from Lap1+/+ and 355 myofibres from Lap1-/- mice were counted. Values are means ± standard errors; **P < 0.001. (F) Frequency histogram showing the distribution of quadriceps muscle fibre CSA in Lap1+/+ and Lap1-/- mice at P2.5; ***P < 0.0001.