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. 2016 Oct 25;26(1):65–78. doi: 10.1093/hmg/ddw368

Figure 7.

Figure 7.

Reduced myogenic potential of satellite cells in skeletal muscle of Myf5-Lap1CKO mice. (A) Cross sections of quadriceps from Control and Myf5-Lap1CKO mice at P5.5 were stained with antibodies against laminin (green) and Pax7 (red). Images were chosen to show areas where Pax7 signals are frequent. Nuclei were counter stained with DAPI (blue). Scale bar: 25 µm. (B) Percentages of Pax7 positive nuclei among total nuclei in quadriceps from Control and Myf5-Lap1CKO mice. Nuclei in (n = 10) images from five different tissue sections from two animals per genotype were counted. Circles are values for individual images and horizontal bars show means; *P < 0.05. (C) Immunoblots of protein extracts of quadriceps from Control (Con) and Myf5-Lap1CKO (CKO) mice at P5.5 and P10.5. Blots were probed with antibodies against indicated proteins. Each lane contained extracts from different mice. (D). Relative expression of mRNAs encoded by Lap1, MyoD, Myf5, Myog (Myogenin), Mef2c and Cdkn1a (p21) in quadriceps from Control and Myf5-Lap1CKO mice at P5.5. Analysis was performed on total RNA from muscles of Control (n = 4) and Myf5-Lap1CKO (n = 4) mice; *P < 0.05, ***P < 0.001, ns: not significant.