Figure 6.

Genetically decreasing sleep in grouped/socially enriched flies activates the UPR. The GAL4 construct P (MB-Switch) was used to drive the UAS-NaChBac construct and decrease sleep in flies. (a) Total sleep (both day and night) was significantly reduced in the NaChBac flies in comparison to their controls; p-values shown on figure. The UPR was induced in the NaChBac flies as indicated by increased (b) BiP expression levels (p < 0.01) and (c) phospho-eIF2α levels (p = 0.016) (n = 6). Mean ± SEM are shown. *p < .05, **p < .01, and ***p < 0.001.