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. 2017 Apr 3;26(12):2299–2306. doi: 10.1093/hmg/ddx121

Figure 4.

Figure 4.

PRKG1 deficiency does not delay retinal degeneration in Pde6g-/- mice. (A) Immunoblots of retinal homogenates from age-matched (2 weeks old) mice of the indicated genotypes. Rod transducin-α (GNAT1) was used as an indicator of rod loss. Actin served as a loading control. (B) Cyclic-GMP stimulated PRKG activity in retinal extracts from the indicated mice (N ≥ 3). One-way ANOVA revealed a significant difference in the group of values (P = 0.0004). Post-hoc Tukey HSD test showed no difference between C57 and Cngb1-/- samples, whereas the value of Cngb1-/-Prkg1-/sample was significantly different from both C57 and Cngb1-/- samples (P < 0.01). (C) Retinal morphology of Pde6g-/- and Pde6g-/-Prkg1-/- littermates at age 2 weeks and 3 weeks. IS: inner segment; ONL: outer nuclear layer; INL, inner nuclear layer. Scale bar = 20 μm. (D) Measurements of outer nuclear layer thickness across the span of the retina at the central meridian (mean ± SD, N ≥ 3). Triangles: Pde6g-/-; diamonds: Pde6g-/-Prkg1-/-. S, superior; I, inferior; O, optic nerve.