Figure 1.

Schematic representation of DAT, NET, and their chimeric constructs. DAT, NET, and their chimeric constructs are presented schematically as having 12 putative transmembrane domains with their COOH and NH₂ termini on the cytoplasmic side of the plasma membrane. Bold and thin lines represent sequences from NET and DAT, respectively. ND-A is a chimeric protein consisting residues 1–128 from NET and 133–620 from DAT. DN-F consists amino acid 1–434 from DAT and the rest from NET (432–617). DATΔnt was constructed by deleting amino acid residues 2–59 from DAT. NntDAT was constructed by attaching the corresponding NET NH₂-terminus (55 residues) to the deletion mutant DATΔnt.