Figure 2.

The NH₂-terminal portion of NET contains a basolateral sorting signal. X-Z cross sections of MDCK cells expressing the transporters were generated by laser scanning confocal microscopy. Each panel is labeled for the transporter construct expressed and the primary antibody used for immunocytochemistry. A diagrammatic representation of the predicted structure of each transporter construct is illustrated in Figure 1. Micrographs for NET and DAT (A–D) are included for reference. Panels B and D are exactly the same fields as A and C, respectively, but cells shown in B and D were stained with an antibody recognizing the Na,K-ATPase α-subunit, a basolateral marker. Bars, 50 μM. Several clonal cell lines or mixtures of cells expressing each transporter construct were examined. The figure shows representative results.