Figure 3.

Alignment of the NH₂ termini from NET and DAT, their chimeric constructs and mutants. The NH₂ termini of DAT, DATΔnt, NntDAT, NETΔ2LL, NntDATm1, NntDATm2, NntDATm3, NntDATm4, NntDATm5, and NntDATm6 are aligned. The amino acid sequence from NET is in bold font and that from DAT is in plain font. The italic font represents regions where NET and DAT have the same sequence. Dashes represent the gaps in the sequence. The last three residues shown are from the first putative transmembrane domain. NETΔ2LL is a NET mutant with two dileucine motifs changed to alanines. DATΔnt was constructed by deleting 59 amino acid residues from DAT. NntDAT was constructed by attaching the corresponding NET NH₂-terminus (55 residues) to the deletion mutant DATΔnt. NntDATm1, NntDATm2, and NntDATm3, are similar to NntDAT but with different portions of the 55-residue NET NH₂-terminus added (residues 1–28, 1–19, and 27–55, respectively). NntDATm1, NntDATm2, and NntDATm3 were generated by site-directed mutagenesis.