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. 2016 Dec 28;26(3):624–636. doi: 10.1093/hmg/ddw421

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Figure 1. — Myosin VIIa interacts with PDZD7. (A) Domain organization of myosin VIIa and PDZD7. Lines below each diagram indicate the myosin VIIa and PDZD7 fragments used in this study. (B) FLAG-myosin VIIa tail pulled down GFP-PDZD7 full-length (FL, lane 7), PDZ1 (lane 8) and PDZ3 (lane 10) proteins, but not GFP-PDZD7 PDZ2 (lane 9) or GFP (pEGFP-C1, lane 6) protein. The lower anti-FLAG blot demonstrates the success of the FLAG pull-down assay. Lanes 1-5 are input samples showing the presence of the transfected proteins in cell lysates. + and -, presence and absence of proteins in the transfected cells, respectively.