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. 2016 Dec 28;26(3):624–636. doi: 10.1093/hmg/ddw421

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Figure 6. — USH2 protein distribution is normal in Ush1c^-/- cochlear hair cells. Immunofluorescent staining of Ush1c^-/- cochleas at P4 demonstrated that ADGRV1 (magenta, A), usherin (magenta, B), whirlin (magenta, C) and PDZD7 (magenta, D) are localized at the base (empty arrows) of stereocilia (phalloidin, green) in IHCs and OHCs, while whirlin is also localized at the stereociliary tip (filled arrows) in IHCs and OHCs. The experiments were performed in 2 litters of Ush1c^-/- pups for ADGRV1 immunostaining, 1 litter for usherin immunostaining, 4 litters for whirlin immunostaining and 2 litters for PDZD7 immunostaining. Scale bars, 1 µm.