Figure 8.
ADGRV1 mislocalization is similar between Pdzd7-/-;Whrnneo/neo and Pdzd7-/- cochlear hair cells. (A) A small amount of ADGRV1 is mislocalized at the stereociliary tip (filled arrows) in about one third of Whrnneo/neo IHCs and OHCs. (B) ADGRV1 is localized either at the stereociliary tips or along the stereocilia as random puncta in Pdzd7-/- IHCs and OHCs. (C) In Pdzd7-/-;Whrnneo/neo IHCs and OHCs, the severity and pattern of ADGRV1 mislocalization are similar to those observed in Pdzd7-/- IHCs and OHCs. One litter of Whrnneo/neo pups, one litter of Pdzd7-/- pups and five litters of Pdzd7-/-;Whrnneo/neo pups were examined. The magenta signals outside the stereociliary bundle are non-specific. Single-channel images of ADGRV1 signals are shown in grayscale on the right of the merged images with the position of arrows the same as that in the merged images. Empty arrows, the base of stereocilia. Scale bars, 1 μm.