FIG. 2.

Effects of the phthalate mixture on the expression of cell cycle regulators. Antral follicles were isolated from mouse ovaries and treated with vehicle (DMSO) or phthalate mixture (1–500 µg/ml) for 96 h. Follicles were collected and subjected to qPCR analyses for the expression of Cdk4 (A), Ccna2 (B), Ccnb1 (C), Ccnd2 (D), Ccne1 (E), Cdkn1a (F), Cdkn1c (G), and Cdkn2a (H). Relative fold changes of each gene normalized to Rn18s are shown. Graphs represent means ± SEM from 3 separate experiments (n = 9–12 follicles/treatment/experiment). Asterisks (*) indicate significant differences from the control (P < .05). ^ indicates borderline significance compared to control, P = .08. M = mixture.