FIG. 6.

Effect of the phthalate mixture on antral follicle-produced sex steroid hormone levels. Antral follicles were isolated from mouse ovaries and treated with vehicle (DMSO) or phthalate mixture (1–500 µg/ml) for 96 h. Following culture, media were pooled per treatment group and were subjected to ELISAs for the measurements of progesterone (A), androstenedione (B), testosterone (C), estrone (D), and estradiol (E). Graph represents means ± SEM from 6 separate experiments, with medium from 6 to 12 wells/treatment group in each experiment. Asterisks (*) indicate significant differences from the control (P < .05). ^ indicates borderline significance compared to control, P = .06. M = mixture.