FIG. 7.

TBBPA induces expression of miR-103 in mouse and human cells. (A) Human adipose-derived MSCs were treated with vehicle (DMSO) or 5 μM TBBPA for 4 days and small RNA was isolated. MiR-103 and miR-107 were detected by qPCR. MiRNA levels were normalized to U6 snRNA. MiR-103 was detected by qPCR. MiR-103/107 levels were normalized to U6 snRNA. (B) 3T3-L1 pre-adipocytes were treated with TBBPA (5 μM) or rosiglitazone (2 μM) with or without adipogenic medium. After 4 days of treatment, miRNA was isolated and miR-103 expression was analyzed using TaqMan qPCR assays. The results shown are a result of three different experiments with three replicate wells measured for each experiment. The data were analyzed by a one-way ANOVA with Tukey’s post-test for significance (*P < .05 and **P < .01).