Fig. 4. Analysis of monomer-dimer distribution of FXIIIA partially activated by thrombin in the presence of 4 mM CaCl₂.

The activation conditions were adjusted so that approximately half of the A-subunits were cleaved. (A) SDS-PAGE (8% gel), monitoring cleavage of FXIIIA by thrombin; (B) theoretic distribution of non-cleaved and cleaved species in the “half-cleaved” samples of FXIIIA. Question marks denote unknown oligomerization state of the “single-cleaved” species. (C) representative c(s) distribution in the 2 μM FXIIIA “half-cleaved” samples with (dashed line) and without (solid line) addition of 5% DMSO. AUC experiments were performed as two replicates for each condition (no DMSO versus 5% DMSO).