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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: FEBS J. 2017 Oct 3;284(22):3849–3861. doi: 10.1111/febs.14272

Fig. 6. Sedimentation velocity analysis of covalently inhibited FXIIIA.

Fig. 6.

2 μM FXIIIA was activated by thrombin in the presence of 4 mM CaCl2 and incubated with inhibitors IAA or K9-DON to form covalent adducts with the active site C314 residue. Samples of inhibited FXIIIA were supplemented with 5% DMSO and subjected to AUC. Structures of IAA and K9-DON are depicted in an insert. Two independent samples were analyzed for each condition.