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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2018 May 19;137:30–39. doi: 10.1016/j.prostaglandins.2018.05.008

Figure 3.

Figure 3.

Db/db mice were fed regular chow (Harlan, Teklad Lab Animals Diets, Indianapolis, IN) and following a 16-week acclimatization period were divided into 3 treatment groups. Group 1 was control. Groups 2 and 3 were injected intraperitoneally with EET-A twice/week for 8 weeks with a dose of 1.5 mg/100g of body weight as previously described (7). The mice in Group 3 were also given 2 bolus injections of 4070 × 106 TU/mouse in 80–100 μΙ PGC-1α (sh) lentiviruses (Dharmacon, Lafayette, CO) into the retro orbital vein. At the end of the experiment, VO2 (A) and VCO2 (B) levels (mL/min/Kg body weight) were determined using the Oxylet gas analyzer. RQ (the respiratory quotient) was calculated as VCO2/VO2 (C). Effect of EET-A treatment on (D) Urinary Sodium normalized to body weight, (E) Protein, Db/db treated with EET-A (black bars/boxes), and PGC-1a-deficient db/db mice treated with EET-A (grey bars/boxes). Results are mean ± SE, n=6, *p<0.05 vs db/db control, #p<0.05 vs db/db mice treated with EET-A alone.