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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 4;441(1):159–175. doi: 10.1016/j.ydbio.2018.07.001

Fig. 10. The ancestry of the robust abdominal epidermis regulatory activity of the CRE controlling Ddc expression.

Fig. 10.

(A–D) A schematic representation of the male abdomen pigmentation patterns for several Sophophora species. (A’–D’) EGFP-reporter transgene expression driven by sequences orthologous to the D. melanogaster Ddc-MEE1 in transgenic D. melanogaster male pupae at the P14-15(i) stage. (A’–C’) Sequences from species with conspicuous melanic pigmentation phenotypes drive reporter expression throughout the abdomen, though the activity appears most pronounced in the A5 and A6 segments. (D’) The sequence from the most distantly-related species with monomorphic tergite pigmentation, D. willistoni, has comparatively less abdominal regulatory activity. Regulatory activity measurements are represented as the % of the wild type D. melanogaster Ddc-MEE1 mean A5 segment intensity plus the Standard Error of the Mean (SEM). Each activity measurement and SEM were derived using images for five biological replicates.