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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 4;441(1):159–175. doi: 10.1016/j.ydbio.2018.07.001

Fig. 5. D. willistoni exhibits little to no Ddc expression in the abdominal epidermis of various pupal stages through eclosion.

Fig. 5.

In situ hybridization for Ddc expression was performed on the dorsal abdominal epidermis of D. willistoni (A–F) male and (A’–F’) female specimens. (A and A’) Ddc is expressed in the mechansosensory bristle cells as early as the P10 stage of pupal development. (B and B’) By the P11 stage, a low level of Ddc expression can be observed in the epidermis regions underlying where tergites will form. (C and C’) By the P12 stage, little Ddc expression is observed in the epidermis regions underlying where tergites will form. (D and D’) At the P13 and (E and E’) P14-P15(i) stages, little to no expression was observed in the epidermis. (F and F’) A Ddc sense probe control shows background signal observed in eclosed flies.