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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 4;441(1):159–175. doi: 10.1016/j.ydbio.2018.07.001

Fig. 6. An abdominal epidermis cis-regulatory element is located 5’ of the endogenous Ddc promoter.

Fig. 6.

(A) To scale representation of the Ddc locus showing regions that were evaluated for CRE activity. (B–I) CRE activity observed by the expression of the EGFP reporter gene in the dorsal abdomens of P14-P15(i) stage transgenic male D. melanogaster pupae. (B and C) UAS-EGFP reporter expression driven by GAL4 expressed under the control of Ddc locus subregions. (B) The entire upstream region of Ddc, named the Ddc-MEE, drove reporter expression in the A2–A6 abdominal regions underlying where tergites develop, a pattern that mimics the endogenous Ddc expression. (C). The 2nd intron region of Ddc drove reporter expression in the mechanosensory bristle cells, a pattern that mimics the endogenous Ddc expression. (D–I) Expression of direct CRE-GFP fusion transgenes. (D) The entire Ddc upstream region drove reporter transgene expression in an abdominal epidermis patter similar to endogenous Ddc expression and that driven by the same region using the GAL4/UAS system. (E) The promoter-proximal Ddc-MEE1 subdivision of the Ddc-MEE retained the patterned abdominal epidermis activity. (F) The Ddc-MEE2 reporter drove expression more broadly in the abdominal epidermis than the Ddc-MEE CRE and endogenous Ddc expression. (G) The Ddc-MEE3 reporter lacks noteworthy abdominal activity. (H and I) Extreme truncated versions of the Ddc-MEE1 region to 364 and 130 base pairs had regulatory activities similar in pattern to that of the Ddc-MEE1 sequence.