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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 4;441(1):159–175. doi: 10.1016/j.ydbio.2018.07.001

Fig. 7. A Grh binding site is a required input in the regulatory logic driving Ddc abdominal epidermis CRE activity.

Fig. 7.

(A) To scale annotation of the Ddc MEE1 region, and the mutant forms of the Ddc-MEE1 that were evaluated for alterations in regulatory activity. (B–E) Regulatory activities of CREs as seen by EGFP reporter gene expression in transgenic D. melanogaster abdomens. (B) Patterned CRE activity driven by the wild type Ddc-MEE1. (C) Compared to the wild type Ddc-MEE1, reporter activity was reduced to 49±3% when the two Grh binding sites were mutated. (D and E) Similarly, the scanning mutant 11 and 12 alterations respectively lowered Ddc-MEE1 reporter activity to 43±5% and 49±7% of the wild type element. Regulatory activity measurements are represented as the % of the wild type D. melanogaster Ddc-MEE1 mean A5 segment intensity plus the Standard Error of the Mean (SEM). Each activity measurement and SEM were derived using images for five biological replicates.