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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Dev Biol. 2018 Jul 4;441(1):159–175. doi: 10.1016/j.ydbio.2018.07.001

Fig. 8. Male-specific pigmentation requires the activity of Grh whose expression has remained conserved.

Fig. 8.

(A) A wild type pattern of pigmentation is exhibited in D. melanogaster males following RNA-interference, RNAi, for the negative control mCherry gene in the dorsal midline pattern of the pnr gene. (A’) Melanic pigmentation was altogether lost in male abdomens following dorsal midline RNAi for the grh gene. Grh protein is expressed in the abdominal epidermis of (B) P12 and (B’) P14-15(i) stage D. melanogaster. Similar patterns of Grh expression occur in (C and C’) P12 and (D and D’) P14-15(i) stage D. willistoni.