Lung injury in post-transition period rat-pups promotes microglia hypo-ramification in the nTS.
A Representative images of coronal brainstem sections from Bleo-treated (n = 7) and saline-treated (n = 6) rats stained for the microglia marker Iba-1.
B The number of microglia in the nTS was not significantly different in Bleo-treated and saline-treated rats (P = 0.431, Two-Tail T-test).
C The number of branch endpoints per microglia decreased in Bleo-treated versus saline-treated rats (**P = 0.0080, Two-Tail T-test).
D The total branch length per microglia decreased in Bleo-treated compared to saline- treated rats (*P = 0.0345).