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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Brain Behav Immun. 2018 Mar 28;70:398–422. doi: 10.1016/j.bbi.2018.03.031

Figure 4.

Figure 4

Lung injury in pre-transition period rats-pups promotes hyper-ramification of Iba-1+ microglia in the nTS and IL-1β/pro-IL-1β production throughout the dorsomedial brainstem.

A Representative images of coronal brainstem sections from saline-treated (black) and Bleo-treated (magenta) groups (7-days after injury) showing immunohistochemical staining for Iba-1+ cells (microglia) in the commissural (SolC), and dorsomedial (SolDM) subnuclei of the nTS. Inset: magnified images showing microglia localized to a medial region of the nTS.

B The number of microglia in the nTS was not significantly different in Bleo-treated (n = 5) or saline-treated (n = 5) rats (P = 0.411, Mean ± SEM, Two-tail t-Test).

C The area of individual microglia from Bleo-treated rats was greater than that of saline-treated rats (*P = 0.018, Two-tail t-Test).

D The number of branch endpoints per microglia increased in Bleo-treated compared to saline-treated rats (*P = 0.038, Two-tail t-Test).

E The total branch length per microglia increased in Bleo-treated compared to saline-treated rats (*P = 0.03, Two-tail t-Test).