Table 1. Primary and secondary antisera used and respective dilutions.
| Antiserum | Dilution | Source | Host species |
|---|---|---|---|
| Primary antisera | |||
| HuC/D Biotin | 1:100 | Invitrogen (16A11) | Mouse |
| VIP | 1: 200 | Immunostar (20077) | Rabbit |
| 5-HT | 1:200 | Immunostar (20079) | Goat |
| CGRP | 1:200 | Immunostar (24112) | Rabbit |
| Substance P | 1:200 | Immunostar (20064) | Rabbit |
| Calbindin (D-28k) | 1:500 | Swant (CB-38a) | Rabbit |
| ChAT | 1:150 | Abcam (ab70219) | Rabbit |
| nNOS (R-20) | 1:50 | Santa Cruz (sc-648) | Rabbit |
| Secondary antisera & streptavidin complexes | |||
| Anti-mouse Alexa Fluor 488 | 1:150 | Molecular Probes (A21202) | Donkey |
| Anti-rabbit Alexa Fluor 488 | 1:250 | Molecular Probes (A21206) | Donkey |
| Cy3-conjugated streptavidin | 1:500 | Amersham (PA43001) | |
| Anti-goat Cy3-conjugated | 1:500 | Jackson ImmunoResearch (705-165-147) |
Donkey |
Supplying companies: Abcam, Cambridge, UK; Amersham, GE Healthcare, Buckinghamshire, UK; Jackson Immuno Research Laboratories, Inc., Baltimore, USA; Molecular Probes, Invitrogen, Carlsbad, CA, USA; Santa Cruz Biotechnology, Inc., CA, USA. Invitrogen, Thermo Fisher Scientific.