Fig 11. Alignment of select TaAOX (hexaploid wheat) proteins with TbAOX (T. brucei).

Yellow highlights indicate conserved motifs. Red font indicates residues proposed to coordinate the diiron center of the active site. Blue font indicates residues experimentally tested for loss of activity by previous researchers. Underlined residues are involved in the TbAOX hydrophobic cavity. Splice variants were identical for the protein region analyzed. The “reg” proteins were not analyzed due to the absence of the conserved motifs.