Table 10. Comparison of residues at dimerization interface between the TbAOX and wheat AOX proteins.
| TbAOX Residue Numbers | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Completely Conserved in TbAOX | Highly Conserved in TbAOX | |||||||||||||||||
| H138◆ | L142 | R143 | R163 | L166 | Q187◆ | M131 | M135 | L139 | S141 | M145 | R147 | D148 | L156 | A159 | M167 | R180 | I183 | |
| TaAOX1a-2AL.sv1 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1a-2AL.sv2 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1a-2BL | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1a-2DL.sv1 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1a-2DL.sv2 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1a-like-2DL | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| TaAOX1c-6AL | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1c-6BL.sv1 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1c-6BL.sv2 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1c-6BL.sv3 | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1c-6DL | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| put.TaAOX1e-3DS | H | L | R | R | L | Q | M | A | L | S | F | Q | S | L | A | M | R | V |
| TaAOX1d-2AL.1 | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| TaAOX1d-2AL.2.sv1 | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| TaAOX1d-2AL.2.sv2 | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| TaAOX1d-2DL | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| put.TaAOX1d-like-4AS | H | L | R | R | L | Q | M | V | L | S | F | H | N | M | A | M | R | V |
| TuAOX1a* | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TuAOX1c* | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| TuAOX1d.1* | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| TuAOX1d.2* | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| AetAOX1a* | H | L | R | R | L | Q | M | M | L | S | F | Q | S | L | A | M | R | V |
| AetAOX1e* | H | L | R | R | L | Q | M | A | L | S | F | Q | S | L | A | M | R | V |
| AetAOX1d* | H | L | R | R | L | Q | M | V | L | S | F | H | S | M | A | M | R | V |
| AetAOX1d-like* | H | L | R | R | L | - | M | V | L | S | F | H | S | M | A | M | - | - |
Completely conserved residues (yellow), highly conserved (green), and semi-conserved (white) in the wheat AOX family are shown. The “reg” proteins were not analyzed due to the absence of the functionally necessary motifs.
*Denotes diploid wheat AOX proteins.
◆Denotes residues which have been experimentally tested.