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. 2018 Aug 3;13(8):e0201926. doi: 10.1371/journal.pone.0201926

Fig 3. Baseline calculation by SVD analysis of the dependence of fluorescence emission on acrylamide concentration.

Fig 3

A: Dependence on acrylamide concentration of fluorescence emission at 340 nm, upon excitation at 280 nm, at 20 ± 0.5°C. The solid line represents a hyperbolic fit of the experimental points (triplicates). B: First two principal spectral components retrieved by SVD analysis (U matrix). C: Dependence on acrylamide concentration of the relative contribution (V matrix) of the first two principal spectral components. D: four higher singular values S of spectral components retrieved by SVD analysis.