Fig 7. Stern-Volmer plots for quenching of SP-B analogue tryptophan fluorescence by 10-doxyl nonadecane.
The ratio of fluorescence emission in the absence and in the presence of 10-DN is plotted as a function of quencher concentration (λex = 280 nm, λem = 340 nm, 20 ± 0.5°C). Experiments were carried out in triplicate and are reported as average ± SE. A: raw data. B: data corrected for inner filter effect according to Leese and Wehry [55].