Table 1. Strand-specific amplification of ZIKV RNA from infected Aedes aegypti larvae.

cDNA	qPCR
	ENV-Fc	ENV-Rd	ENV-F+Re
ENV-Ra	32.5*	Negative	32.2*
ENV-Fb	Negative	33.4*	33.3*

Two different cDNA were produced with ENV-Reverse^a primer or ENV-Forward^b primer and RNA extracted from the infected larvae pool. The qPCR master mixes were made only with forward primer (ENV-F^c); only with reverse primer (ENV-R^d) or with both primers (ENV-F+R^e).

* Median Ct values from qPCR experiments in triplicate. Amplification of the negative-strand RNA is highlighted in bold.