Skip to main content
. 2018 Aug 3;13(8):e0201936. doi: 10.1371/journal.pone.0201936

Fig 1. Experimental design.

Fig 1

5/6 Nx = 5/6 nephrectomy, Ctrl = control, CRF = chronic renal failure, HP = high phosphate, NP = normal phosphate, D = alfacalcidol, ICG = ICG-001, Veh = Vehicle, 8w = 8weeks. After four weeks of uremia CRF rats were allocated to five experimental groups according to weight and plasma urea. CRF-D rats were treated with alfacalcidol to establish VC. At eight weeks the alfacalcidol was stopped and subsequently rats received daily injections of either ICG-001 or vehicle for four weeks. Rats were sacrificed after 12 weeks of uremia. A group of alfacalcidol treated CRF rats was sacrificed at 8 weeks to examine the alfacalcidol-induced mineral metabolism disturbances at baseline. CRF rats not treated with alfalcalcidol and Ctrl rats were allocated to four weeks of either ICG-001 or vehicle and sacrificed after 8 weeks.