Fig 2. Plasma biochemistry.

Plasma levels of (a) creatinine, (b) urea, (c) intact fibroblast growth factor 23 (iFGF23), (d) PTH, (e) Ca²⁺ and (f) phosphate measured at sacrifice. CRF and CRF-D rats had a mild to moderate reduction in kidney function with increased levels of plasma creatinine, urea, iFGF23 and PTH. In the CRF-D rats sacrificed at 8 weeks the iFGF23 levels were extremely elevated, PTH was suppressed, and a trend towards an increase in Ca²⁺ was seen. iFGF23 levels were slightly lower in ICG-001-administered Ctrl compared to vehicle-administered Ctrl and plasma phosphate was markedly higher in the ICG-001-administered CRF compared to the vehicle-administered CRF. Data is presented as mean ± SD, and PTH as median and interquartile range. n = 6–9. Vehicle-administered Ctrl, CRF and CRF-D rats were compared by one-way ANOVA and Dunnets multiple comparison with *P<0.05, **P<0.001 and ***P<0.0001 vs Ctrl. ICG-001- and vehicle-administered groups were compared using two-tailed t-test with ^#P <0.05 vs vehicle.