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. 2018 Aug 3;13(8):e0201936. doi: 10.1371/journal.pone.0201936

Fig 3. Kidney gene and protein expression.

Fig 3

Kidney gene expression of factors related to fibrosis and EMT as well as Wnt signalling was examined in Ctrl rats (a) and in CRF rats (c). In Ctrl rats ICG-001 resulted in a decrease in the expression of Snai1 (snail1), Vim (vimentin), Ctnnb1 (β-catenin), Ccnd1 (cyclinD1) and Tgfb1 (TGF-β), while these effects were eliminated in CRF rats. Protein levels of total β-catenin (TBC), active β-catenin (ABC) and cyclinD1 were examined by WB in kidneys from Ctrl (b) and CRF rats (d). In accordance with the gene expression results, protein levels of active β-catenin and cyclinD1 were reduced in kidneys from ICG-001-administered Ctrl rats while no differences in protein levels were seen between ICG-001- and vehicle-administered CRF rats. Blots cropped from different parts of the same gel are presented together. Uncropped blots are provided in S2 Fig. Data is presented as mean ± SD. n = 6–9. *P<0.05 vs Ctrl by unpaired two-tailed t-test, #P <0.05 vs vehicle by unpaired two-tailed t-test.