Fig 9. Social interaction tests using three-room chamber.

In a sociability test SPF and GF mice were not different in time spent in chamber with a strange mouse (A) or with an empty cage (B) at either four (n = 15 for both female and male SPF mice; n = 6 for female and n = 9 for male GF mice) or 12 (n = 13 for both female and male SPF and n = 7 for both female and male GF mice) weeks of age. In the test for social novelty, no difference between SPF and GF groups was found in time spent with a familiar mouse (C), but there was a significant decrease in time spent with a novel mouse in GF mice at 12 weeks (D). Asterisks indicate significant differences when p-value is at least <0.05.