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. 2018 Jul 10;7:e38268. doi: 10.7554/eLife.38268

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© 2018, Akbergenova et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 8. — (A) Representative serial time points of NMJ development visualized by repeated imaging through the cuticle of an anesthetized larvae at the indicated time points beginning at the early first instar stage. Two of the five brightest PSDs present during the first imaging session are labeled and are the first to develop the peripheral GluRIIB segregation pattern 36 hr later. GluRIIB labeling alone is shown in the bottom panel. The right panels show GluRIIB fluorescence and normalized GluRIIA and GluRIIB fluorescent line profiles for the indicated PSDs at the initial imaging session (0 hr) and 36 hr later. (B) Serial images of an NMJ with a larger number of AZs present at the first instar stage. After 36 hr of development, the peripheral segregation of GluRIIB around GluRIIA was first observed in some of the PSDs that were present during the initial imaging session (numbered). The right panels show GluRIIB fluorescence and normalized GluRIIA and GluRIIB fluorescent line profiles for the indicated PSDs at the initial imaging session (0 hr) and 36 hr later. The dashed box surrounds the actual imaged segment of the NMJ in each panel.

Figure 8—figure supplement 1. — (A) Representative serial time points of NMJ development visualized by repeated imaging through the cuticle of an anesthetized larvae at the indicated time points beginning at the early first instar stage. Two of the five brightest PSDs present during the first imaging session are labeled and are the first to develop the peripheral GluRIIB segregation pattern 36 hr later. GluRIIB labeling alone is shown in the bottom panel. The right panels show GluRIIB fluorescence and normalized GluRIIA and GluRIIB fluorescent line profiles for the indicated PSDs at the initial imaging session (0 hr) and 36 hr later. (B) Serial images of an NMJ with a larger number of AZs present at the first instar stage. After 36 hr of development, the peripheral segregation of GluRIIB around GluRIIA was first observed in some of the PSDs that were present during the initial imaging session (numbered). The right panels show GluRIIB fluorescence and normalized GluRIIA and GluRIIB fluorescent line profiles for the indicated PSDs at the initial imaging session (0 hr) and 36 hr later. The dashed box surrounds the actual imaged segment of the NMJ in each panel.