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. 2018 Jul 16;138(3):266–282. doi: 10.1161/CIRCULATIONAHA.117.030856

Figure 6.

Figure 6.

Enhanced aortic root atherosclerosis in Apoe–/–LmnaG609G/G609G and Apoe–/–LmnaLCS/LCSSM22αCre mice is accompanied by inflammation of adjacent cardiac tissue and plaque destabilization. Mice were fed a high-fat diet for 8 weeks starting at 8 weeks of age. Serial sections of the aortic root were made and 3 different regions were stained with Masson trichrome. A and B, Representative photographs of atheromata in the aortic root of Apoe–/–LmnaG609G/G609G mice (A) and Apoe–/–LmnaLCS/LCSSM22αCre mice (B), and their corresponding controls. Graphs show quantification of plaque area, with each point representing the mean for 3 aortic root regions; n=5. Scale bar: 200 µm. Dashed lines indicate medial perimeter (the last layer of elastin) and dotted lines indicate the luminal surface of the atheroma plaque. C, An example of intraplaque (Top) and intravalve (Bottom) hemorrhage in Apoe–/–LmnaG609G/G6090G mice. Scale bar: 100 µm. D, An example of intraplaque hemorrhage (Top) and thrombus formation (Bottom) in Apoe–/–LmnaLCS/LCSSM22αCre mice. Scale bar: 100 µm. Data are shown as mean±SEM. Statistical analysis was performed by 2-tailed t test. **P<0.01. ***P<0.001.