Temporal dynamics of DAF-16 iscep-1dependent. (A) Representative images of DAF-16::GFP in N2, cep-1, ercc-1 and cep-1 ercc-1 worms at D1 and D9 of adulthood. Green arrows indicate nuclear localization of DAF-16::GFP and white arrows indicate lack thereof. (B-C) Percent survival of D1 or D9 adult N2, cep-1, ercc-1 and cep-1 ercc-1 worms 16 h after treatment with rotenone (mean ± S.E.M., n = 4 groups of 40–60 worms/genotype; 3 replicas). Student's two-tailed t-test * *p < 0.01; ns not significant.