Table 1.
List of In Vitro-Transcribed Modified Cas9 Cap 1 mRNAs Made with CleanCap
| Abbreviation | Full Name | Uridine Depleted | Cap Form | Screen |
|---|---|---|---|---|
| WT UD | wild-type bases | yes | Cap 1 | full |
| 5moU UD | 5-methoxy uridine | yes | Cap 1 | full |
| Ψ UD | pseudouridine | yes | Cap 1 | full |
| WT | wild-type bases | no | Cap 1 | full |
| 5moU | 5-methoxy uridine | no | Cap 1 | full |
| Ψ | pseudouridine | no | Cap 1 | full |
| 5meC/Ψ | 5-methyl cytidine/pseudouridine | no | Cap 1 | full |
| 5meU | 5-methyl uridine | no | Cap 1 | full |
| N1-me-Ψ | N1-methyl pseudouridine | no | Cap 1 | full |
| 5meC | 5-methyl cytidine | no | Cap 1 | full |
| 5hmC | 5-hydroxymethyl cytidine | no | Cap 1 | full |
| N1-et-Ψ | N1-ethyl pseudouridine | no | Cap 1 | initial |
| me1-Ψ /5meC | N1-methyl pseudouridine/5-methyl cytidine | no | Cap 1 | initial |
| 5moC | 5-methoxy cytidine | no | Cap 1 | initial |
| 5camU | 5-carboxy methyl ester uridine | no | Cap 1 | initial |
| 10% 5meC/2sU | 5-methyl cytidine/2-thio uridine | no | Cap 1 | initial |
| 25% 5meC/2sU | 5-methyl cytidine/2-thio uridine | no | Cap 1 | initial |
| ARCA 5meC/Ψ | 5-methyl cytidine/pseudouridine | no | Cap 0 | initial |