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Mass spectrometry analysis of S-glutathionylated Cys37 and Cys118. ask1Q27K recombinant protein was incubated with 10 µM GSNO for 15 min and analyzed by MS after tryptic digestion. MS/MS spectra of S-glutathionylated (A) ask1Q27K Cys37 or (B) Cys118 peptide is shown. Fragmentation patterns that correspond with ions of the y (blue) and the b (red) series are included as inserts.
