Skip to main content
. 2018 Jun 21;10:28–39. doi: 10.1016/j.omto.2018.06.002

Figure 4.

Figure 4

Infection of TNF-α-Armed Oncolytic VSV Induces Bystander Cell Death of SMC-Treated Cancer Cells

(A) Confluent monolayers of EMT6 and CT-26 cell were overlaid with agarose media containing vehicle or 5 μM of the SMC LCL161 and inoculated with 500 PFU of virus in the middle of the well for 48 hr. Cytotoxicity was assessed by crystal violet. Graph represents the percentage cytotoxicity (mean, SD). B, EMT6 and SNB75 cells were infected with 1 MOI of the indicated virus for 24 hr. The supernatant was exposed to UV light, and then the supernatant was applied at the indicated dilutions to new EMT6 or SNB75 cells in the presence of vehicle or 5 μM LCL161 for 48 hr. (C) Conditioned media from splenocytes was generated as in (B) and applied to EMT6 cells in the presence of vehicle or 5 μM LCL161 for 48 hr. (D) SNB75 cells were transfected with combinations of nontargeting (NT), TNF-R1, and DR5 siRNA for 48 hr and treated with vehicle or 5 μM LCL161 and infected with the indicated virus for 48 hr. (E) EMT6 cells were pretreated with 50 μg/mL of TNF-α neutralizing antibody or isotype IgG control for 1 hr and subsequently treated with 5 μM LCL161 and 1 MOI of the indicated virus. (B–E) Cell viability was assessed by Alamar blue. Mean, SD.