Extended Data Figure 9 |. Sleep/wakefulness behavior of Nalcn mutant mice.

a, Representative 8s-EEG and EMG for wake, NREMS and REMS of Nalcn mutant mice b, Representative hypnogram of Nalcn^+/+ mice (upper) and Nalcn^Drl/+ mice (lower). Wake (blue), NREMS (green) and REMS (red) are indicated from ZT0 to ZT12. c, Enlarged hypnogram of around ZT7 showed the frequent transitions between NREMS and REMS of Nalcn^Drl/+ mice, d, Total wake time and NREMS time of Nalcn^Drl/+ mice (n = 29) and Nalcn^+/+ mice (n = 25). Wake, P = 0.58; NREMS, P = 0.17, One-way ANOVA. e-f, Circadian period length (e) and amplitude of circadian behavior (f) in constant darkness of Nalcn^Drl/+ mice (n = 6) and Nalcn^+/+ mice (n = 7). Two-tailed Student’s t-test, (e) P = 0.76. (f) *** P < 0.001. g, Total REMS time of Nalcn^Drl/+ mice (n = 9) and Nalcn^+/+ mice in constant darkness (n = 8). Two-tailed Student’s t-test. *** P < 0.001. h, EEG power spectra of Nalcn^Drl/+ mice (n = 29) and Nalcn^+/+ mice (n = 25). One-way ANOVA followed by Tukey’s test. *** P < 0.001. Values are means ± sem.