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. 2018 Aug 3;8:11682. doi: 10.1038/s41598-018-30143-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative muscle biopsies: COX/SDH staining and ddPCR plots. (A) Longitudinal biopsy section. (B) Transverse biopsy section with COX negative cell labelled as 1. (C) RBF cell labelled as 2. (D) Intermediate cell labelled as 3 and COX positive cell labelled as 4. (E) Example of ddPCR 1D plot where each droplet from a sample is plotted on the graph of fluorescence intensity vs. droplet number. All positive droplets, blue for FAM or green for HEX, are scored as positive and each is assigned a value of 1. All negative droplets (grey), are scored as negative. (F) The histogram which plots amplitude vs. the frequency of the populations of droplets.