Fig. 3.

Treacle impacts HeV production and interacts with wt but not K258A HeV M protein. a HeLa cells were transfected with scrambled siRNA (scr), Treacle siRNA, or mock-transfected, before infection with HeV (MOI 0.5); siRNA for polo-like kinase 1 (PLK1) is a positive control known to inhibit HeV production¹⁷. HeV titer was measured at 48 h p.i. (mean TCID₅₀/mL, ± s.e.m., n = 3). Statistical analysis used Student’s t-test; **, p = 0.003. b IPs of the indicated proteins from HEK-293T cells were analyzed by immunoblotting (IB) with the indicated antibodies; results are representative of three independent experiments. c HeV-infected cells were fixed 24 h p.i. and immunostained for HeV M protein and Treacle. Nuclei were labeled using Hoechst 33342 (blue in merged image). Images are representative of ≥20 fields of view, capturing >100 cells over two experiments. d HeLa cells transfected to express the indicated proteins were fixed and immunostained for Treacle; images are representative of ≥30 fields of view over three experiments. Scale bars correspond to 15 μm