Fig. 7.

HeV M protein expression inhibits rRNA production. HeLa cells were transfected to express the indicated GFP-fused proteins before treatment with or without ActD for 1 h (a, b), or DMSO or etoposide (3 h) (c, d), and labeling of nascent RNA as described in the legend to Fig. 6 (fixation at 16 h p.t.; histogram shows mean nucleolar EU fluorescence ± s.e.m.; n ≥ 31 nucleoli, data from a single assay, representative of six independent assays for b; n ≥ 39 nucleoli, data from a single assay, representative of three independent assays for d). e, f HeLa cells were transfected with scrambled (scr) or Treacle siRNA followed by transfection to express GFP-HeV M protein and labeling of nascent RNA (fixation 16 h p.t.; histogram shows mean nucleolar EU fluorescence ± s.e.m., n ≥ 23 nucleoli, data from a single assay representative of three independent assays). Statistical analysis used Student’s t-test; * p < 0.05; **, p < 0.01; *** p < 0.001; NS, Non-significant. Scale bars correspond to 15 μm