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. 2018 Aug 3;8:11674. doi: 10.1038/s41598-018-30046-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autophagic cell death assessment in MCF-7 (A) and T47D (B) cells after exposure to GCB, TQ and their combination. Cells were exposed to GCB, TQ or their combination for 24 h; and were stained with Cyto-ID autophagosome tracker. Net fluorescent intensity (NFI) were plotted and compared to basal fluorescence of control group. Data is presented as mean ± SD; n = 3. *Significantly different from control group. **Significantly different from GCB treatment.