Figure 9.

SEM and CLSM images of RBL-2H3 cells. IgE-sensitized RBL-2H3 cells were stimulated with DNP-BSA in the presence of active compounds derived from ume seed at 600 μM or vehicle (0.1% DMSO). (a) Cells were fixed, dehydrated and lyophilized. Cells were then coated with palladium and platinum and visualized by SEM. (b) Cells were fixed and stained with Acti-stain 488 phalloidin. Cells were visualized by CLSM. Red arrows indicate cells with membrane ruffling. (c) The percentage of cells with membrane ruffling was calculated from 5–7 SEM images. **p < 0.001, compared with antigen-stimulated vehicle.