Fig. 4.

The incorporation of the envelope glycoproteins is related to the cell surface expression levels, not the stoichiometry of F/M or G/M. VLPs described in Fig. 3h were subjected to confocal microscopy imaging. The VLPs were located by the GFP signal from M; the intensities of the F and G signals co-localized with M were analyzed. a, b Histograms of F (a) and G (b) incorporation levels in VLPs collected at 18 (gray) and 45 (red) h post transfection of F and G. c, d Histograms of F/M (c) and G/M (d) ratios in VLPs collected at 18 (gray) and 45 (red) h post transfection of F and G. e Expression levels of F (top) and G (bottom) in the GFP-positive and VLP-producing cells at 18 (gray) and 45 (red) h post tranfection. Cells expressing M alone were set as control (blue dotted line). Three independent experiments were performed