Figure 5.

CRB2 distribution in the mouse cortex. (A–B) Immunofluorescence CRB2 labeling of a sagittal section of the adult WT (A) and CRB1^rd8. (B) Cortex of the mouse brain showing the protein distribution throughout the layers of the cortex. (C) Graph showing the quantification of the CRB2 relative labeling/px in the different layers of the cortex of WT and CRB1^rd8 mice. (D) Graph showing the normalized quantification of the CRB2 relative labeling/px in the whole CRB1^rd8 mouse cortex related to WT. (E–F) WB analysis of the expression of CRB2 in adult WT and CRB1^rd8 mice brain. (E) Comparative CRB2 (150 kD) protein expression in WT and CRB1^rd8 mice cortex, hippocampus and cerebellum areas. Blots were cropped from the same gel and quantitative comparisons were performed between samples of the same blot. Full-length blots are presented in Supplementary Fig. 4. (F) Graph obtained from data in E showing the comparative quantification of CRB2 protein expression in WT and CRB1^rd8 mice cortex, hypothalamus and cerebellum. β-actin (42 kD) was used as the loading control. DAPI (in blue): nuclear labeling. Scale bars: 200 µm. Data are presented as mean ± s.e.m. Statistical information: The Kolmogorov Smirnov test was used to assess the normality of sample distribution. In C-WT, more than three groups were analyzed with one way-factorial ANOVA and the Bonferroni’s post-hoc test (n = 3). In C-rd8, more than three groups were analyzed with Kruskal Wallis test (n = 3). In D, two experimental groups were compared with the Student’s T test (n = 3). In F, in all cases, two experimental groups were compared with the Mann Whitney U test (n = 15). Asterisks indicate statistical differences. Two asterisks indicate highly statistical differences.