Figure 3.

NAGLU Corrected N-iNSC Grafting after 2 Months

(A) Annotated image of the parameters for intracerebroventricular (ICV, green) and intraparenchymal (PAR, blue) injections. X indicates represented injection sites. (B) Immunohistological staining of 40-μm representative sections for GFP, shown in low magnification, following ICV and intraparenchymal transplantion of N-iNSCs at birth (cells have a GFP reporter) in Naglu^−/− mice 2 months after transplantation. Post-acquisition processing included adjustments to brightness and contrast and red green blue (RGB) curves using Adobe Photoshop CS6 to improve visibility and consistency in color tone. Scale bar, 1 mm. Rostrocaudal distribution of N-iNSCs relative to the level of greatest engraftment is shown in millimeters (bottom left corner). FACS analysis showing percentage engraftment (C and D, top) and NAGLU enzyme activity assays are shown in units per milligram of protein (C and D, bottom), performed on brain lysates following ICV and intraparenchymal injection (n = 6 in each treatment).