Figure 6.

Correction of Neuropathology and Storage Accumulation in Naglu^−/− Mice Treated with Neural Stem Cells Overexpressing NAGLU N-iNSCs at 9 Months

(A–C) Representative bright-field images of half-brain coronal sections from heterozygous control mice (unaffected) and mice injected with saline (−/− vehicle) or N-iNSCs (−/− treated). Images were taken at three levels along the rostrocaudal axis (rostral, middle, and caudal) and immunohistochemically stained for CD68 (A), GFAP (B), and Lamp 1 (C), respectively. Numbered boxes show images taken at higher magnification from three specific areas within each section (boxes 1–3, outlined at lower magnification, top). The same three areas were analyzed for GFAP and Lamp 1. Post-acquisition processing was applied to all images and included adjustments to brightness and contrast and RGB curves using Adobe Photoshop CS6 to improve visibility and consistency in color tone. Scale bars, 1 mm.